By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this publication covers such issues as environmental overview of biotechnological methods and microbial changes of haloaromatic and haloaliphatic compounds.
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Additional info for Advances in Applied Microbiology, Vol. 3
Fry and Greaves (1951) showed that very young ( 4 to 6 hour) cultures of their paracolon bacillus seemed to be far more sensitive to drying than older (18 to 25 hour) cultures. Naylor and Smith (1946) found that S. mrcescens grown at 30° to 34OC. for 18 to 24 hours was more resistant to drying than cultures grown for longer or shorter periods. Benedict et al. (1958) not only confirmed the general observations of Naylor and Smith, but showed that, with constant aeration, cultures incubated at 29O or 3OOC.
Heckly (1947) described a lyophilizing apparatus which was similar in some respects to Flosdorfs glass unit but it required less glass blowing skill. The condenser was a Pyrex serum bottle into which was fitted a standard taper joint, through a rubber stopper so that various manifolds could be conveniently interchanged. A tube for evacuating the systems was sealed through the side of the standard joint since the insertion of two tubes through a rubber stopper distorted the stopper sufficiently to cause leaks.
Hammer (1911), Rogers (1914), Swift (1937), Greaves (1956), and Elser et al. ( 1935) have described modifications of a method originally reported by Shackell (1909) who used an ordinary laboratory desiccator. The modifications were concerned primarily with the method of freezing the samples, the choice of desiccant, or the techniques of operation. They employed concentrated sulfuric acid, phosphorus pentoxide, or calcium sulfate, which was placed in the bottom of a desiccator to absorb moisture, and various methods were utilized to freeze the samples and to keep them frozen during the operation.