Biometals by G. Winkelmann

By G. Winkelmann

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06 vs. 23 vs. 001, respectively). STZ administered rats had significantly higher concentrations of both glucose and the early glycation product GHb than the control group (205 vs. 65 vs. 001, respectively). These findings reflected the establishment of diabetes mellitus in STZ administered rats. 9 vs. 01). 3 vs. 1 vs. 8 vs. 05, respectively). 1 vs. 01). Pearson correlation analysis results are shown in Table 2. 05). 05) (Figs. 1, 2). 05) (Figs. 05). 05 level 123 Biometals (2010) 23:43–49 47 AGE-peptides and oxidative stress related parameters in diabetic and nondiabetic states.

25 mol/l hydrochloric acid. The mixture was heated for 30 min in a boiling water bath. After cooling, the flocculent precipitate was removed by centrifugation at 1000 g for 10 min. 56 9 105 M-1 cm-1. Assay of glutathione (GSH) GSH was measured in erythrocyte lysate. Erythrocytes were separated and washed three times in 5 ml of sterile 9 g/l NaCl solution and hemolyzed by diluting four-fold with water. Erythrocyte GSH concentration was determined according to the method of Beutler et al. (1963) using metaphosphoric acid for protein precipitation and 5,50 dithiobis 2-nitrobenzoic acid for color development.

2006) since higher concentrations of zinc, from 50 to 1,000 lM, decreased the signal. 5 to 25 lM. However, our conclusions differ from those of Femling et al. (2006), since it seems that higher concentrations of zinc promote the conversion of O2•- into other reactive species beyond H2O2. According to Irato and Albergoni (2005) a greater availability of zinc leads to decrease in the catalase activity. We speculate that this inhibition of catalase augmented H2O2 levels, which then may be rapidly transformed in other reactive species.

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