By Peter J. A. Bollen, Axel Kornerup Hansen, Helle J. Rasmussen, P. J. A. Bollen
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Infections which may pass with the organ from the animal donor to the human recipient. More information about the risk of porcine retroviruses for humans is needed to evaluate the risk connected with porcine-human xenotransplantation. It is obvious that porcine infections already known to possess a zoonotic potential should be a matter of concern. However, these are well-defined and diagnostic and preventive tools are welldeveloped, while there is little knowledge of the risks connected with the xenotransplantation entry port.
1999 CRC Press LLC microbiological sampling63,92 Blood samples for serology are taken from the jugular vein or cranial caval vein. Usually, not more than a few drops of blood can be taken from ear veins, but this may be sufficient for some tests. Methods of blood sampling are described in Chapter 5. Sterile, cotton-tipped swabs should be used for sampling nasal secretions. The swab is introduced into one of the nares, and inoculated onto a plate or kept in Stuart's transport medium for bacterial culture.
Under 20%, the population size is of some impect on the sample size. If this is not the case, the sample size (S) can be 83 calculated from following equation : S > log C. (1-(p*N1)) C= accepted risk of a false negative diagnosis; N1= sensitivity of the applied test; p= expected prevalence Staphylococcus hyicus, S. 87 SPF swine do not harbour infections such as A. pleuropneumoniae and Mycoplasma hyopneumoniae, which are mentioned in the guidelines for this category of swine, but concerning nonlisted agents there is no guarantee for the absence of the other abovementioned agents.